Application

Anti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research CategoryInflammation & Immunology

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Chitinase-1 (CHIT1) in human spleen and bone marrow tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chitotriosidase-1 (UniProt: Q13231; also known as EC: 3.2.1.14, Chitinase-1) is encoded by the CHIT1 gene (Gene ID: 1118) in human. Chitinase-1 is a member of the Chitinase class II subfamily that degrades chitin, chitotriose and chitobiose. It is one of the most abundantly secreted protein and is mainly produced by activated macrophages and epithelial cells. Chitinase -1 can hydrolyze the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. It may also participate in the defense against nematodes and other pathogens. Four isoforms of Chitinase-1 have been described that are produced by alternative splicing and isoform 3, which lacks amino acids 344-372, does not possess any enzymatic activity. Chitinase-1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved to generate the mature form. Its chitin-binding domain is located in amino acids 417-466 and has three chitooligosaccharide binding regions (aa 7-71; 97-100; and 210-213). Elevated levels of Chitinase-1 are observed in cases of Gaucher′s disease, bronchial asthma, and atherosclerosis. (Ref.: Kanneganti, M., et al. (2012). J. Epithel. Biol. Pharmacol. 5; 1-9).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chitinase-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in human serum.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Chitinase-1 in human serum.

Specificity

This rabbit polyclonal antibody detects Chitinase-1 in human serum. It targets an epitope within 15 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).